The strike by members of the Swedish Dockworkers Union began at 2pm on Tuesday and is due to run until Friday evening.
"We did not want to end up in this situation," union representative Peter Annerback told TT news agency. "We would not have done this if we had any other choice. We do not think our demands are unreasonable."
Unlike with the Swedish Transport Workers' Union, the Dockworkers Union does not have a collective agreement with Swedish ports and so are not bound by a no strike rule.
APM Terminals, which owns the port, said the strike would have consequences for trade as around half of the container ship traffic around Sweden uses the port in Gothenburg, TT reported.